At 13H43 Monday afternoon Netcare 911 responded to reports of a serious collision on the N3 near the Barry Marais offramp in Vosloorus.
Reports from the scene indicate that multiple vehicles including trucks were involved in a collision.
When paramedics arrived on scene they found patients lying all over the road.
Paramedics assessed the scene and found that nine people had sustained fatal injuries and at least another twenty five patients sustained injuries ranging from minor to critical.
All the patients were treated on scene by advance life support personnel and once stabilised were transported by various ambulance services to hospital for further treatment.
Circumstances leading up to the collision will be investigated by the relevant authorities who were on scene.